产品详细描述
概述
Product Name: Biotinylated conjugated Goat anti-Mouse IgG (H+L)
Catalogue Number: 511-012 -1
LotNumber: See the label
Amount: 0.2 mg in 0.2 ml PBS, pH 7.4 with stabilizer
Appearance: Sterile liquid.
Storage Condition:
Keep the product at 2--8o C with sterilecondition. For long term storage, add an equal amount ofglycerol, mix and stored at –20oC. Alternatively, theproduct may be aliquoted and stored at -70oC or belowwithout adding glycerol. Avoid freezing and thawing frequently.
Expirationdate: Six-month from date of receiving.
ProductsSpecifications:
The antigen affinity purified antibody is labeled by Biotin-SP.The antibody reacts with the heavy chains on Mouse IgG and with thelight chains common to most mouse immunoglobulines. No antibody wasdetected against non-immunoglobulin serum proteins. It hasminimal reaction to human serum protein.
Application:
The Goat anti-Mouse IgG (H+L) antibody can be used for ELISA,Western blot, Immunohistochemistry. Other immunossays are nottested. All the working solutions need to be made freshly. Theworking concentrations for specific applications should bedetermined by researchers. The following factors need to beconsidered when an experiment is performed: 1) detection methods,2) antigen form, 3) experiment conditions, etc.
Suggested dilution range:
Application | Dilution | Recommendation |
ELISA | 1:5000-1:20,000 | Chromogenic substrate |
Western Blotting | 1:1000-1:10,000 | ECL substrate |
Immunohistochemistry | 1:500-1:5000 | Tissue section |